Journal: BioMed Research International
Article Title: High Hydrostatic Pressure Therapy Annihilates Squamous Cell Carcinoma in a Murine Model
doi: 10.1155/2020/3074742
Figure Lengend Snippet: The specimens at nine weeks after injection of inactivation of cSCC cells by HHP. (a) The gross appearance of the back of mice nine weeks after cSCC cell injection without pressurization (control) on the left side and with pressurization (pressurized group) on the right side. Scale bars = 1 cm. (b, c) HE staining, (d, e) p63 immunohistochemical staining, and (f, g) Ki-67 immunohistochemical staining of the specimens injected with cSCC cells without pressurization ((b, d, f) control group) and with pressurization ((c, e, g) pressurized group). Scale bars = 250 μ m. (h) The percentage of Ki-67-positive cells in each slide was calculated using an image analysis software program. ∗∗ P < 0.01.
Article Snippet: The Ki-67-positive cells (control group: n = 10, pressurized group: n = 7) were identified using the Hybrid Cell Count image analysis program (Keyence Corp.)
Techniques: Injection, Control, Staining, Immunohistochemical staining, Software